Autophagy, much like regular car maintenance, is a vital intracellular cleaning process that prevents the accumulation of toxic materials within cells. At its core, ‘autophagic flux’ signifies the speed at which these harmful substances are efficiently removed through autophagy. Inadequate autophagy has been closely linked to accelerated ageing and the early onset of age-related ailments such as diabetes, cancer, and notably, Alzheimer’s disease. Therefore, accurately measuring autophagy activity holds immense significance.